Procurement Summary: Melatonin (MEL-RES-027)

Focus: Concise Summary for Procurement

This document provides a summary of the Melatonin reagent, Catalog ID MEL-RES-027, for use by the procurement department.

Compound Identification and Overview

Catalog ID: MEL-RES-027

Compound: Melatonin (N-acetyl-5-methoxytryptamine)

Overview A naturally occurring indoleamine produced by the pineal gland. Functions as a potent antioxidant, circadian rhythm regulator, and mitochondrial protectant. Widely used in research to study sleep physiology, oxidative stress, and cellular aging.

Key Specifications

• Form: Crystalline Powder
• Mass: 232.3 Da
• Purity: >99.1%
• Storage: -20°C (Protect from light).

Safety and Handling

Safety Research Reagent. Buyer assumes all responsibility for safe handling and proper disposal.

This section concludes the initial summary requested for procurement. The following pages serve to expand the document to the requested length of 10 pages while maintaining a professional focus relevant to research procurement.

Section 2: Detailed Chemical and Structural Information

Molecular Formula and Structure

The chemical structure of Melatonin is defined by the formula $C_{13}H_{16}N_{2}O_{2}$. It is an indoleamine derivative, characterized by the methoxy group at position 5 and the $N$-acetyl group at position 3 of the ethylamine side chain.

Characteristic

Detail

Chemical Name

N-acetyl-5-methoxytryptamine

Molecular Weight

232.28 g/mol

CAS Number

73-31-4

Appearance

White to Off-White Crystalline Powder

Reagent Quality Assurance

All batches of MEL-RES-027 are subject to rigorous quality control protocols. Purity is determined by High-Performance Liquid Chromatography (HPLC) and Liquid Chromatography-Mass Spectrometry (LC-MS).

Potential Applications in Research

Melatonin is a critical tool in multiple fields of biological research:

1. Chronobiology: Studying the regulation of the sleep-wake cycle and circadian rhythms through the MT1 and MT2 receptors.
2. Oxidative Stress: Investigating its role as a direct free-radical scavenger, particularly against reactive oxygen and nitrogen species.
3. Mitochondrial Function: Research into its protective effects on mitochondrial membrane potential and ATP synthesis.
4. Neuroscience: Exploring its potential therapeutic role in neurodegenerative diseases.

Section 3: Storage, Handling, and Stability Protocol

Storage Requirements (MEL-RES-027)

Strict adherence to storage conditions is essential to maintain the integrity and purity of the reagent.

• Primary Storage: -20°C freezer, in original sealed container.
• Light Sensitivity: Highly sensitive to light degradation. The container must be shielded from all light exposure, especially UV light.
• Desiccation: Should be stored under dry conditions to prevent moisture absorption.

Safe Handling Procedures

All laboratory personnel handling MEL-RES-027 must wear appropriate Personal Protective Equipment (PPE).

PPE Requirement

Description

Hand Protection

Nitrile or rubber gloves

Eye Protection

Safety goggles or face shield

Respiratory Protection

N/A (Use in a fume hood if powder dispersion is likely)

Work Area

Chemical fume hood or designated safety enclosure

Stability Data Summary

Melatonin is generally stable when stored according to the specified conditions. Stability tests show that under optimal storage at -20°C, the product maintains >99% purity for [placeholder text: time frame] from the date of manufacture.

Section 4: Regulatory and Disposal Information

Research Use Only Statement

MEL-RES-027 is classified as a "Research Use Only" (RUO) chemical.

• It is not intended for human or animal therapeutic use.
• It is not intended for diagnostic use.
• It is not intended for food, drug, or cosmetic use.

Material Safety Data Sheet (MSDS)

The current Material Safety Data Sheet (MSDS) for Melatonin (MEL-RES-027) is available for download: File. All users must review this document prior to initial handling.

Waste Disposal Protocol

All unused or spent Melatonin must be disposed of as hazardous chemical waste.

1. Neutralization: Check the MSDS for recommended neutralization protocols; generally not required for this product.
2. Collection: Collect waste in a properly labeled, sealed container designated for non-halogenated organic solids.
3. Contractor: Coordinate pick-up with authorized hazardous waste disposal contractor, Person.

Section 5: Procurement Logistics and Inventory

Packaging and Lot Sizing

MEL-RES-027 is available in standard research-grade unit sizes.

Standard Size

Container Type

Storage Temperature

100 mg

Amber Glass Vial

-20°C

500 mg

Amber Glass Bottle

-20°C

1 g

Amber Glass Bottle

-20°C

Ordering and Lead Time

All purchase orders should reference the Catalog ID: MEL-RES-027.

• Standard Lead Time: 5-7 business days from order confirmation.
• Rush Orders: Available upon request; contact Person.

Inventory Control Considerations

Due to its strict storage requirements (-20°C and light protection), inventory stock of MEL-RES-027 should be minimized to ensure the freshest product is always utilized in experiments. Inventory review should occur monthly on Date.

Section 6: Research Context and Mechanistic Summary

Melatonin's primary action involves binding to the MT1 and MT2 G-protein coupled receptors, which are involved in regulating the circadian cycle.

• MT1 Receptor: Inhibition of adenylyl cyclase, leading to decreased cAMP and subsequent modulation of neuronal firing.
• MT2 Receptor: Involved in phase-shifting the circadian clock.

Beyond receptor binding, its non-receptor-mediated functions as a direct antioxidant are critical in cellular studies.

Section 7: Recommended Analytical Methods

For labs requiring in-house verification of the reagent, the following analytical methods are recommended.

High-Performance Liquid Chromatography (HPLC)

Parameter

Recommended Setting

Column

C18 Reverse-Phase

Mobile Phase

Acetonitrile/Water

Detection

UV at 278 nm

Flow Rate

1.0 mL/min

Spectrophotometric Analysis

Melatonin exhibits a characteristic UV absorption maximum ($\lambda_{max}$) at approximately 278 nm in methanol or ethanol, which can be used for concentration determination ($\epsilon_{278} \approx 6,300 M^{-1}cm^{-1}$).

Section 8: Troubleshooting and Quality Issues

Common Quality Concerns

If a batch of MEL-RES-027 is suspected of having reduced purity or stability:

1. Check Storage Log: Verify that the product was continuously maintained at -20°C and protected from light.
2. Solvent Purity: Ensure the solvent used for dissolution (typically ethanol or DMSO) is fresh and of high purity.
3. Contact Support: Immediately notify the vendor quality control team at File.

Section 9: Related Products and Future Procurement

Other Indoleamine Reagents

Research frequently requires related compounds for comparative studies. Future procurement may include:

• Serotonin
• $N$-Acetylserotonin
• Tryptophan

Compound

Catalog ID

Note

Serotonin

SER-RES-001

Precursor molecule

$N$-Acetylserotonin

NAS-RES-002

Immediate precursor to Melatonin

Projected Usage and Reorder Point

Based on current research demand, the projected annual usage of MEL-RES-027 is [placeholder text: quantity]. The reorder point should be set when stock falls below 200 mg to account for lead time.

Section 10: Documentation and Contact Information

Essential Documentation Links

For full transparency and compliance, all relevant documents are linked below:

• Certificate of Analysis (COA): File
• Safety Data Sheet (SDS): File
• Specifications Sheet: File

Key Contact Information

For procurement and technical inquiries regarding MEL-RES-027:

Role

Contact Person

Email Address

Procurement Lead

Person

[placeholder text: email address]

Technical Support

Person

[placeholder text: email address]

Safety & Disposal

Person

[placeholder text: email address]

The next review of this procurement summary is scheduled for the Calendar event, which is on Date.