Fragment 176–191

Introduction and Classification

Fragment 176–191 is a modified synthetic polypeptide used exclusively for in vitro and in vivo laboratory research. It is a derivative of human Growth Hormone (hGH) designed to isolate and enhance the metabolic effects associated with the C-terminal region of the native hormone.

Product Name

Fragment 176–191 - Research Grade

Origin

C-Terminal Human Growth Hormone Derivative

Classification

Modified AOD9604 Analog

Product Description

Fragment 176–191 is a synthetic peptide corresponding to the C-terminal amino acid residues 176 through 191 of human Growth Hormone (hGH). This specific sequence, containing 16 amino acids, has been engineered to be a non-growth-promoting analog of hGH.

In laboratory research, it is commonly known as the "lipolytic fragment" due to its ability to mimic the potent, localized fat-burning (lipolytic) properties of hGH. Crucially, the structure of Fragment 176–191 is designed to achieve these effects without stimulating Insulin-like Growth Factor 1 (IGF-1) secretion or inducing the hyperglycemia often associated with full-length hGH administration.

Its mechanism of action is believed to involve a specific interaction with hGH receptors on adipose tissue, stimulating lipolysis (the breakdown of fat) and inhibiting lipogenesis (the formation of fat). It is a valuable tool for studying metabolic pathways, obesity, and lipodystrophy in various research models.

Specifications

The following table details the quality and physical characteristics of the Fragment 176–191 peptide supplied for research purposes.

Parameter

Specification

Form

Lyophilized Powder

Sequence

C-Terminal hGH residues 176-191

Molecular Formula

[Chemical Formula Placeholder]

Molecular Weight

[Molecular Weight Placeholder]

Purity

>99% (HPLC)

Counter Ion

Acetate

Appearance

White to Off-White Powder

Solubility

Soluble in water/saline (Bacteriostatic Water recommended for reconstitution)

Handling, Storage, and Preparation

Lyophilized Powder Storage

The lyophilized powder form of Fragment 176–191 is highly stable.

• Temperature: Store at -20°C for long-term storage.
• Conditions: Keep container tightly sealed and protected from light and moisture.
• Shelf Life: Expected stability for 24 months under recommended storage conditions.

Reconstitution (Preparation of Stock Solution)

Reconstitution should be performed under sterile conditions suitable for cell culture or in vivo administration.

1. Preparation: Allow the vial to warm to room temperature prior to opening.
2. Solvent: Reconstitute with a suitable sterile solvent, such as Bacteriostatic Water for Injection (BWFI) or sterile 0.9% Saline.
3. Mixing: Slowly add the solvent to the vial wall. Do not inject directly onto the powder. Gently swirl the vial; avoid shaking vigorously to prevent peptide degradation.
4. Concentration: Typically reconstituted to a concentration between 1 mg/mL and 5 mg/mL, depending on experimental requirements.

Storage of Reconstituted Solution

Once reconstituted, the peptide solution has reduced stability.

• Temperature: Store at 4°C.
• Shelf Life: Stable for up to 7 days when stored correctly. For longer storage (up to 4 weeks), aliquot the solution and freeze at -20°C.
• Freeze-Thaw Cycles: Minimize freeze-thaw cycles, as they can reduce peptide integrity.

Research Applications

Fragment 176–191 is employed in various research settings focused on metabolic disorders and signaling pathways.

Key Research Areas

1. Lipolysis and Adipose Tissue: Used to study the direct mechanisms by which Growth Hormone fragments stimulate the breakdown of triglycerides in white adipose tissue.
2. Metabolic Syndrome and Obesity: Investigating its potential role as a metabolic modulator to reduce fat accumulation in animal models without the side effects of full hGH.
3. Glucose Homeostasis: Used as a negative control against full-length hGH to study the differential effects of specific hGH regions on insulin sensitivity and blood glucose levels.
4. Hormone Receptor Signaling: Studying the specific receptor binding characteristics of the C-terminal fragment on hGH receptors compared to the N-terminal and full-length sequences.

Safety and Regulatory Disclaimer

FOR LABORATORY RESEARCH USE ONLY.

Fragment 176–191 is a research chemical. It is not approved for human consumption, therapeutic use, veterinary use, or as a food supplement. The material is supplied strictly for scientific research and development purposes.

Hazard Information

Researchers must follow standard laboratory practices when handling this material. A Material Safety Data Sheet (MSDS) is available upon request and is essential for safe handling.

• Personal Protective Equipment (PPE): Wear appropriate gloves, lab coat, and eye protection.
• Inhalation: Avoid breathing dust/fume/gas/mist/vapors/spray. Use in a well-ventilated area or fume hood.
• Disposal: Dispose of product and container in accordance with local, regional, national, and international regulations.

Responsibility

The researcher is solely responsible for determining the suitability of Fragment 176–191 for their intended research application and for adhering to all safety protocols and regulatory guidelines, including securing any required permits.

References and Documentation

Further technical data and quality assurance documentation can be found in the accompanying files.

• Certificate of Analysis (CoA): File
• High-Performance Liquid Chromatography (HPLC) Report: File
• Mass Spectrometry (MS) Report: File
• Material Safety Data Sheet (MSDS): File

Ordering Information

For pricing, lead times, and bulk orders, please contact the supplier’s sales department at [Email Address Placeholder] or call Person at [Phone Number Placeholder].

The next major research conference detailing peptide research will be the Calendar event, held at Place on Date.

Product Code

Unit Size

Price (USD)

F176-191-001

1 mg

[Price Placeholder]

F176-191-005

5 mg

[Price Placeholder]

F176-191-010

10 mg

[Price Placeholder]